Immunohistochemical expression of Fas/Fasligand and c-Myc in oral lichen planus
Immunohistochemical expression of Fas/Fasligand and c-Myc in oral lichen planus
Blog Article
Background: Oral Lichen Planus is a chronic inflammatory mucosal disease, presenting in various clinical forms.Both antigen-specific and non-specific mechanisms involved in the pathogenesis of OLP.Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms.
Fas is a cell-surface glycoprotein, 40-kDa, that belongs to the nerve growth factor / tumor necrosis factor (TNF) receptor family.Fas is expressed in several tissues including blood, where its expression is upregulated on activated T and B lymphocytes and natural killer cells.Fas ligand is a type II transmembrane protein that belongs to the tumor necrosis factor family.
The proto-oncogene c-Myc is a transcripation factor with roles in cellular proliferation, differentiation, apoptosis and cell cycle progression.Mutation in the c-Myc gene and protein over-expression has been associated with moonbon a variety of hematopoietic tumors, leukemias and lymphomas.Apoptosis is the mechanism that would be dysregulated in this disease.
This study was conducted to evaluate the expression of Fas, Fasl and c-Myc in oral lichen planus and to correlate the expression of either markers with each other.Materials and Methods: This study was performed on thirty formalin- fixed paraffin-embedded tissue blocks of oral lichen planus pro and retrospectively.An immunohistochemical staining was done by using monoclonal antibodies for Fas, Fasl and c-Myc.
Results: Expressions of Fas, Fasl and c-Myc were highly detected in keratinocytes and inflammatory cells of OLP cases compared to negative expression in normal oral mucosa.Significant correlation has been found between expression of Fas, Fasl and c-Myc in epithelial cells with that of inflammatory cells in oral lichen planus studied cases.Significant correlation has been found among expressions of Fas, Fasl and c-Myc in epithelial cells of oral lichen planus cases.
Significant positive correlation found between expressions of Fas, Fasl and c-Myc in keratinocytes and inflammatory cells of oral lichen planus.Conclusion: Increased expression level of Fas, Fasl and c-Myc in both keratinocytes and lymphocytes of OLP cases in comparison to normal mucosa with highly vip-130al significant correlation among the markers expression indicate their important role in malignant transformation of oral lichen planus.